Springer Nature
12861_2016_114_MOESM3_ESM.tif (917.98 kB)

Additional file 3: Figure S2. of Mediator subunit Med12 contributes to the maintenance of neural stem cell identity

Download (917.98 kB)
posted on 2016-05-17, 05:00 authored by Nam Kim, Carolina Livi, P. Yew, Thomas Boyer
Validation of Med12 and Cdk8 depletion in representative knockdown experiment. Nuclear extracts from mNS-5 NSCs infected with lentiviruses expressing non-specific (NS) control or Med12-specific shRNAs were harvested and resolved by SDS-10 % PAGE prior to processing by immunoblot using antibodies specific for Med12, Cdk8, the p89 subunit of transcription factor IIH (p89), the latter of which served as an internal loading control. (TIF 917 kb)


National Institute of Mental Health