Additional file 3: Figure S2. of Genome-wide transcriptional regulation of estrogen receptor targets in fallopian tube cells and the role of selective estrogen receptor modulators

FVB MOE response to E2 and SERMs. a, b) qPCR analysis of (a) Pgr and (b) Greb1 induction in response to 48 h hormone starvation and 24 h treatment with solvent, 1 nM E2 or 100 nM SERMs. “a” indicates significant difference compared to all treatments. c) Relative growth of FVB MOE following 72-hour hormone starvation and 72-hour treatment with solvent, 1 nM E2, 100 nM SERMs and the combination as monitored by SRB assay. d) Relative migration of FVB MOE cells following 72-hour hormone starvation and 24-hour treatment with solvent, 1 nM E2 or 100 nM SERMs. Wound initiated at time of treatment. e) qPCR analysis of Nrip1 expression levels in CD1 and FVB MOE cells in response to 24 h treatment with DMSO or 1 nM E2. (TIF 615 kb)