12929_2018_479_MOESM2_ESM.tif (833.69 kB)

Additional file 2: of Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology

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posted on 08.11.2018, 05:00 by Yi-Chao Lee, Wan-Chen Huang, Jiann-Her Lin, Tzu-Jen Kao, Hui-Ching Lin, Kuen-Haur Lee, Hsin-Chuan Lin, Che-Kun Shen, Wen-Chang Chang, Chi-Chen Huang
Figure S2. (A) 293 T cells transfected with Flag-Znf179 or Flag-Znf179-5A mutant for 48 h were then treated with 10 μM MG132 or 10 mM 3-MA for 4 h. Total cell lysates were analyzed by Western blotting with anti-Znf179 and anti-β-actin antibodies. (B) Protein intensity were quantified by AlphaEase FC software and the statistic results were presented as mean ± s.e.m. of at least three independent experiments (*, P < 0.05). (TIF 833 kb)


Taipei Medical University Hospital