13395_2020_252_MOESM2_ESM.ai (21.28 MB)
Additional file 2 of Tetraspanin CD82 is necessary for muscle stem cell activation and supports dystrophic muscle function
figureposted on 2020-11-27, 04:51 authored by Arielle Hall, Tatiana Fontelonga, Alec Wright, Katlynn Bugda Gwilt, Jeffrey Widrick, Alessandra Pasut, Francesco Villa, Cynthia K. Miranti, Devin Gibbs, Evan Jiang, Hui Meng, Michael W. Lawlor, Emanuela Gussoni
Additional file 2: Supplementary Figure 2. The number of Pax7+ satellite cells does not differ between WT and CD82-/- animals. Upper panels show single stained and merged representative immunofluorescence images of TA muscle tissue sections from non-injured (WT and CD82-/-) and cardiotoxin-injured muscles 7 days following injury (WT CTX7D and CD82-/-CTX7D) mice. Sections were immunostained for Pax7 (green) dystrophin (red) and DAPI (blue) as indicated. Arrows point to nuclei of Pax7+ cells in each panel. Quantification of the number of Pax7+ cells/myofiber from uninjured muscle and from regenerating muscle show no significant difference between the genotypes. Scale bars=50 microns. Lower panels show single myofibers isolated from EDL muscles of WT and CD82-/- mice immediately fixed and stained for Pax7 expression (red). Quantification of the number of Pax7+ cells per single myofibers between WT and CD82-/-show no significant difference (n=4/genotype).