Additional file 2 of Tetraspanin CD82 is necessary for muscle stem cell activation and supports dystrophic muscle function

Additional file 2: Supplementary Figure 2. The number of Pax7+ satellite cells does not differ between WT and CD82-/- animals. Upper panels show single stained and merged representative immunofluorescence images of TA muscle tissue sections from non-injured (WT and CD82-/-) and cardiotoxin-injured muscles 7 days following injury (WT CTX7D and CD82-/-CTX7D) mice. Sections were immunostained for Pax7 (green) dystrophin (red) and DAPI (blue) as indicated. Arrows point to nuclei of Pax7+ cells in each panel. Quantification of the number of Pax7+ cells/myofiber from uninjured muscle and from regenerating muscle show no significant difference between the genotypes. Scale bars=50 microns. Lower panels show single myofibers isolated from EDL muscles of WT and CD82-/- mice immediately fixed and stained for Pax7 expression (red). Quantification of the number of Pax7+ cells per single myofibers between WT and CD82-/-show no significant difference (n=4/genotype).