Additional file 2: Figure S2. CD25 and Foxp3 expression in splenocytes from littermate and LAT mice. (A) FACS analysis of CD4, CD25, and Foxp3 in splenocyte from 6-week-old littermates and LAT mice. Dead cells were gated out by using fixable viability dye. FACS plot is a representative of analysis of six mice (littermate, n=3; LAT, n=3). (B) The proportion of CD4+CD25+Foxp3+ cells were compared between littermates and LAT mice. *p<0.05, Unpaired t test. The data are presented as means ± SEMs.