Additional file 2: of MOV10 binding circ-DICER1 regulates the angiogenesis of glioma via miR-103a-3p/miR-382-5p mediated ZIC4 expression change

Figure S2. The transfection efficiency of shcirc-DICER1, miR-103-3p/miR-382-5p, ZIC4 and Hsp90β, and miRNA microarrays data. (A) The transfection efficiency of circ-DICER1 knockdown was detected by qRT-PCR. Data represent means ± SD (n = 5, each group). **P < 0.01 versus. Circ-DICER1 (−) NC group. (B) The expression of DICER1 was measured after knockdown of circ-DICER1. Data represent means ± SD (n = 5, each group). (C) The mRNA expression of DICER1 was detected by qRT-PCR after DICER1 knockdown. Data represent means ± SD (n = 5, each group). **P < 0.01 versus. sh-DICER1. (D) The expression of circ-DICER1 was measured after knockdown of DICER1. Data represent means ± SD (n = 5, each group). (E) MiRNA gene expression profiles as obtained from samples in three groups as indicated. (F) The binding sites between DICER1 and miR-103a-3p were predicted, and the relative luciferase activity was evaluated in HEK293T cells. Data represent means ± SD (n = 5, each group). **P < 0.01 versus DICER1 Wt + miR-103a-3p (+) NC group. (G-H) The transfection efficiency of miR-103a-3p (G) and miR-382-5p (H) agomir or antagomir were evaluated by qRT-PCR. Data represent means ± SD (n = 5, each group). **P < 0.01 versus. miR-103a-3p / miR-382-5p (+) NC group, ##P < 0.01 versus. miR-103a-3p / miR-382-5p (−) NC group. (I) The transfection efficiencies of ZIC4 were evaluated with western blot. Data represent means ± SD (n = 5, each group). **P < 0.01 versus. ZIC4 (+) NC group, ##P < 0.01 versus. ZIC4 (−) NC group. (J) The transfection efficiency of Hsp90β was investigated with western blot. Data represent means ± SD (n = 5, each group). **P < 0.01 versus. Hsp90β (+) NC group, ##P < 0.01 versus. Hsp90β (−) NC group. (TIF 780 kb)