Additional file 2 of Long non-coding RNA OIP5-AS1 suppresses multiple myeloma progression by sponging miR-27a-3p to activate TSC1 expression
2020-05-08T04:00:11Z (GMT) by
Additional file 2: Fig. S2. Overexpression of OIP5-AS1 hampered proliferation and metastasis, but facilitated apoptosis of MM RPMI-8226 cells. MM RPMI-8226 cells were transfected with LncRNA OIP5-AS1 or Lnc-NC. (A) The relative expression of lncRNA OIP5-AS1 in transfected cells was analyzed by qRT-PCR assay. (B) Cell viability of transfected cells was analyzed by CCK-8 assay. (C) BrdU incorporation in transfected cells was determined by BrdU staining assay. (D) The colony formation ability of transfected cells was monitored via colony formation assay. (E–F) The apoptosis rate and cell cycle distribution were tested through flow cytometry assay. (G-H) The migration and invasion abilities of transfected cells were examined by transwell assay. (I) The protein levels of Cleaved caspase 3/total caspase 3, Cleaved caspase 1, γ-H2AX, Cyclin D1, p21, Ki-67, MMP9, MMP7 and MMP10 were evaluated by western blot analysis. *P < 0.05.