Additional file 2 of IL-23 in arthritic and inflammatory pain development in mice

Additional file 2: Figure S2. IL-23 is required for optimal mBSA-induced synovial macrophage response. (A-C) Analysis of neutrophils and macrophages from naïve and mBSA-injected joints (day 1) of WT and Il23p19-/- mice. (A) Representative FACS plots showing the gating strategy used to identify synovial neutrophils (CD11b+ Ly6G+) (R1) and macrophages (CD11b+ F4/80+) (R2); (B-C) numbers of total cells (CD45+), neutrophils (R1) and macrophages (R2) in (B) naïve and (C) mBSA-injected joints (day 1) from WT and Il23p19-/- mice. (D-E) Analysis of blood monocytes and neutrophils from naïve WT and Il23p19-/- mice. (D) Representative FACS plots showing the gating strategy used to identify monocytes (CD11b+ CD115+) (R3) and neutrophils (CD11b+ Ly6G+) (R4); (E) numbers of total blood leukocytes, monocytes (R3) and neutrophils (R4). Data are expressed as mean ± SEM; (B-C) WT and Il23p19-/- female mice (saline/mBSA n=10), (E) WT and Il23p19-/- female mice (n=6). For statistical analysis, an unpaired Student’s t-test was used. *p<0.05, WT vs. Il23p19-/- mice.