Additional file 2: of G-quadruplex ligand RHPS4 radiosensitizes glioblastoma xenograft in vivo through a differential targeting of bulky differentiated- and stem-cancer cells

Figure S2. Analysis of the telomere status in U251MG-Adh and -Sph cells. Telomere lengths were analyzed using centromere-calibrated QFISH. Representative images of metaphase spreads from U251MG-Adh and -Sph cells. (A) Distributions of telomere length in U251MG-Adh and U251MG-Sph (B and C). Box plot in C reports means, quartiles and s.d. (n = 2). Fragile telomere frequency in U251MG-Adh and U251MG-Sph. Box plot reports means, quartiles and s.d. (n = 2) (D). Telomerase activity (TA) in both parental and stem-like derived cells. Data represent mean values ± s.d. (n = 2) (E). ** P < 0.01, *** P < 0.001 (Student’s t-test). (TIF 17312 kb)