Additional file 2 of Enrichment differentiation of human induced pluripotent stem cells into sinoatrial node-like cells by combined modulation of BMP, FGF, and RA signaling pathways

Additional file 2: Suppl-Fig. 2. Evaluation of the ventricular and atrial working cardiomyocytes in the BMP4/PD/BMS (BPM)-induced SANLC population. (A, B) Compared to the GiWi control, the expressions of specific atrial markers (MYL7 and COUPTFII) (A) and ventricle markers (MYL2 and NKX2.5) (B) were remarkably downregulated in BPM enriched SANLC at day 16 indicated by qPCR analysis (t test, * p < 0.05 versus GiWi control, n = 3). (C) Representative IF analysis further showed that quite low fractions of COUPTFII+and MLC2V+ (corresponding MYL2 gene) population was observed in total cells indicated by DAPI. Scale bars, 100 μm (400×). Expression values of all PCR analyses were normalized to the housekeeping gene GAPDH. Data are presented as ‘Mean ± SD’.