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Additional file 2 of Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system
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posted on 2020-06-24, 03:36 authored by Remi L. Gratacap, Tim Regan, Carola E. Dehler, Samuel A. M. Martin, Pierre Boudinot, Bertrand Collet, Ross D. HoustonAdditional file 2 Figure S2. Editing efficiency estimation, The analysis of the editing of pooled cell population samples using ICE online software. a. The chromatograms (.ab1 file) from the control (non-edited) and edited samples, along with the gRNA sequence are uploaded on ice.sythego.com. b. The platform verifies that the cut site corresponds to the start of the mixed population chromatogram and deconvolutes the picks to original sequences + or – a few bases. c. The results are presented as the percentage of each edited sample present in the pooled population contributing to the mixed chromatogram.