Additional file 2: of Comprehensive comparison of three different animal models for systemic inflammation

Total glutathione concentration in different organs, glutathione-S-transferase activities and F4/80 expression in the livers. At the time point indicated, mice were sacrificed and different organs were collected for the analysis of the total glutathione content (a-d). Furthermore, glutathione-S-transferase (GST) activities were determined in the 9000 g liver supernatants (e). Data are given as mean ± standard deviation (SD); n = 4–6 for each group and time point. Statistical significance was determined by using the non-parametric Kruskal-Wallis test, followed by pairwise Mann-Whitney U tests. Statistical comparisons were made versus the control of each group and are denoted as follows: LPS (asterisk, *), PCI (plus, +), CLP (diamond, #). A p value <0.05 (*,+,#) was considered statistically significant; a p value <0.01 (**,++,##) and a p value <0.001 (***,+++,###) are further specified. The photomicrographs in (f) show representative livers after 48 h, displaying large amounts F4/80 positive cells after LPS and PCI treatment. In (g), HE stainings of PCI- and CLP-treated mice after 24 h are shown as a supplement to Fig. 3d. PCI and CLP treatment caused almost no fat accumulation in the livers. (TIFF 1084 kb)