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Additional file 2 of Chorionic villus-derived mesenchymal stem cell-mediated autophagy promotes the proliferation and invasiveness of trophoblasts under hypoxia by activating the JAK2/STAT3 signalling pathway

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posted on 14.10.2021, 03:27 by Yijing Chu, Chengzhan Zhu, Chongyu Yue, Wei Peng, Weiping Chen, Guifang He, Changchang Liu, Yang Lv, Guoqiang Gao, Ke Yao, Rendong Han, Xiaoyu Hu, Yan Zhang, Yuanhua Ye
Additional file 2: Figure S2. Effect of PE-CV-MSC CM on the proliferation, invasion, autophagy and JAK2/STAT3 signalling of trophoblasts. (A) Representative CCK-8 assay results in JAR, JEG-3 and HTR-8 cells are shown. Trophoblast cells were treated with PE-CV-MSC CM under hypoxic conditions. (B) The number of trophoblasts that migrated through the 8-μm Transwell membrane pores was counted to determine changes in the invasive capabilities in response to CV-MSC CM under hypoxic conditions. (C) STAT3, IL-6 and LIF mRNA levels were determined in JAR, JEG-3 and HTR-8 cells treated with PE-CV-MSC CM by qRT-PCR. (D) Protein expression of p-JAK2, p-STAT3, LC3 II, BECN1, P62 and MMP2/9 was examined in JAR, JEG-3 and HTR-8 cells treated with PE-CV-MSC CM by western blotting.

Funding

Clinical Medicine+X Project of the Affiliated Hospital of Qingdao University national natural science foundation of china young taishan scholars program of shandong province postdoctoral science foundation of china

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