Additional file 2: of A novel bladder cancer - specific oncolytic adenovirus by CD46 and its effect combined with cisplatin against cancer cells of CAR negative expression

The identified results of Ad5/F11p-PSCAE-UPII-E1A by PCR method. PSCAE gene, UPII gene, and E1A gene express in the recombinant adenovirus of Ad5/F11p-PSCAE-UPII-E1A and the control adenovirus Ad5-PSCAE-UPII-E1A were identified by PCR method. Gene expression bands were observed in agarose gel electrophoresis. The lanes 1 and 5 are bands of marker. The lanes 2, 3 and 4 are gene bands of PSCAE, UPII, and E1A of Ad5/F11p respectively, and the lanes 6, 7 and 8 are gene bands of PSCAE, UPII, and E1A of Ad5 respectively. The molecular sizes of marker are 100 bp, 200 bp, 300 bp, 400 bp, 500 bp, 700 bp, and 1000 bp respectively (from the bottom up). The molecular sizes of PSCAE gene, UPII gene, and E1A gene are 327 bp, 314 bp, and 541 bp respectively. (TIFF 17684 kb)