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Additional file 2: Figure S2. of The impact of sequence database choice on metaproteomic results in gut microbiota studies
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posted on 2016-09-27, 05:00 authored by Alessandro Tanca, Antonio Palomba, Cristina Fraumene, Daniela Pagnozzi, Valeria Manghina, Massimo Deligios, Thilo Muth, Erdmann Rapp, Lennart Martens, Maria Addis, Sergio UzzauThe influence of taxonomic filter level on the 16S-based (16S, top) and proteomic iterative (PI, bottom) databases. Venn diagrams indicate overlap among non-redundant peptide identifications obtained using UniProt sequences belonging to all families (F), genera (G), or species (S) detected after 16S rDNA gene sequencing and QIIME taxonomic assignment (16S; taxon abundance threshold >0.1Â %), or a preliminary search against UniProt Bacteria and peptide taxonomic assignment using Unipept (PI; taxon abundance threshold >0.5Â %). Peptide identifications were obtained at 5Â % FDR, using three different bioinformatic platforms (left, MetaProteomeAnalyzer; middle, MaxQuant; right, Proteome Discoverer) with the corresponding (and above indicated) search engines and peptide validation tools. (TIF 1580 KB)