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Additional file 2: Figure S2. of Soluble oligomeric amyloid-β induces calcium dyshomeostasis that precedes synapse loss in the living mouse brain
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posted on 2017-03-21, 05:00 authored by Michal Arbel-Ornath, Eloise Hudry, Josiah Boivin, Tadafumi Hashimoto, Shuko Takeda, Kishore Kuchibhotla, Steven Hou, Carli Lattarulo, Arianna Belcher, Naomi Shakerdge, Pariss Trujillo, Alona Muzikansky, Rebecca Betensky, Bradley Hyman, Brian BacskaiTime course of oAβ-induced increase in resting calcium levels after acute exposure with TgCM on a healthy brain. C57BL/6 mice were injected with AAV-CBA-YC3.6 into the somatosensory cortex and acutely exposed to TgCM containing oAβ. The changes in YFP/CFP ratios in the neurites were measured at baseline before treatment and 30 min, 1 h and 5 h after topical application of TgCM. A progressive increase in the levels of resting calcium (YFP/CFP ratio and the actual calcium levels on the lower and upper x-axis, respectively) was detected that reached a peak after 1 h (20.6% of overloaded neurites), before returning to baseline levels after 5 h (1.7% overloaded neurites). (TIF 37844 kb)