Additional file 2: Figure S2. of Contribution of the non-effector members of the HrpL regulon, iaaL and matE, to the virulence of Pseudomonas syringae pv. tomato DC3000 in tomato plants

Determination of the transcription start sites of the iaaL and matE genes in P. syringae pv. tomato DC3000. Total RNA of P. syringae pv. tomato DC3000 grown on minimal media was extracted. (A) For iaaL, runoff cDNAs were generated by extending primers R161 and R121 (Table S1). Lanes A, C, G, and T contain the products of the dideoxy sequencing reactions with using primer R161. Arrows indicate the size of the runoff cDNAs obtained for each primer. (B) Nucleotide sequence of the intergenic region including the 3′ and 5′ ends of the matE and iaaL ORFS, respectively. (C) For matE, the +1 position was determined by 5′RACE and subsequent sequencing. B and C: The coding sequences of both genes are shadowed. The transcription start sites (P iaaL/matE) are marked with a black spot and are shown in bold type; the arrowhead indicates the direction of transcription. The hrp box motifs are shown in gray boxes, and the sequences of the primers used are underlined. (TIFF 3064 kb)