Additional file 2: Figure S1. of Long intergenic non-coding RNA 00152 promotes lung adenocarcinoma proliferation via interacting with EZH2 and repressing IL24 expression

LINC00152 expression decreased after LINC00152 silencing. (a) LINC00152 expression in A549 and SPCA1 cells transfected with three discrete chemically synthesized siRNAs. (b) LINC00152 expression in H1299 cells transfected with pcDNA3.1-LINC00152 vector. (c) Western blotting analysis of bcl-xl protein level in A549 and SPCA1 cells, GAPDH protein was used as an internal control. (d) EZH2 expression in A549 and SPCA1 cells transfected with si-EZH2. (e) Western blotting were performed to detect the EZH2 and LSD1 protein level in A549 and SPCA1 cells. (f) Western blotting analysis of IL24 protein level in A549 and SPCA1 cells, GAPDH protein was used as an internal control. Values are shown as the mean Âą s.d in three independent experiments. *P < 0.05, **P < 0.01. (TIF 6891 kb)