Additional file 1 of USP9X-mediated REV1 deubiquitination promotes lung cancer radioresistance via the action of REV1 as a Rad18 molecular scaffold for cystathionine γ-lyase
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posted on 2024-05-28, 04:03 authored by Yunshang Chen, Xue Feng, Zilong Wu, Yongqiang Yang, Xinrui Rao, Rui Meng, Sheng Zhang, Xiaorong Dong, Shuangbing Xu, Gang Wu, Xiaohua JieSupplementary Material 1: Fig. S1. REV1 expression can be effectively knocked down by shRNAs and siRNAs. A The mRNA levels of the indicated molecules were measured by qRT-PCR in shControl and shREV1 lung cancer cells. *** P < 0.001 (n = 4). B REV1 knockdown cell line was successfully constructed by using shRNA technology (n = 3). C The mRNA levels of the indicated molecules were measured by qRT-PCR in scramble and siREV1 lung cancer cells.*** P < 0.001 (n = 4). D A549 cells transfected with the indicated siRNAs were harvested and analyzed by Western blotting (n = 3).
