13064_2020_144_MOESM1_ESM.tif (199.07 kB)
Additional file 1 of The first quarter of the C-terminal domain of Abelson regulates the WAVE regulatory complex and Enabled in axon guidance
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posted on 2020-05-03, 04:00 authored by Han Sian Joshua Cheong, Mark Nona, Samantha Barbara Guerra, Mark Francis VanBerkumAdditional file 1 Figure S1. Expression levels of Abl transgenes and Gal4 drivers. (A) Expression levels of Abl transgenes, as assessed by western blots from 3rd instar CNS expressing transgenes with 1407-Gal4. (B) Comparison of expression levels of 1407-Gal4 and elav-Gal4 at 2 temperatures. The drivers were used to drive expression of UAS-RFP in the CNS of 3rd instar larvae. All immunoblotting was carried out against the C-terminal FLAG tag of the transgenic proteins, and are representative of three replicates.
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SH 3 domainsactin dynamicsactin regulator Enabledactin regulator EnaWRCCTD2 EC-terminal domainPxxP motifResults GST pulldownMethods Protein binding partners1 Q binding partners1 Q regionaxon guidanceRemoving 1 Eaxon guidance assays1 QAbl transgenes2 E regionaxon guidance Abstract Background Abelson tyrosine kinase
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