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Additional file 1 of RBM45 associates with nuclear stress bodies and forms nuclear inclusions during chronic cellular stress and in neurodegenerative diseases

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posted on 2020-06-26, 03:53 authored by Mahlon Collins, Yang Li, Robert Bowser
Additional file 1: Figure S1. Assessment of RBM45 association with cytoplasmic stress granules (SGs). HEK293 cells were stained for RBM45 and the SG marker protein G3BP. (a) In untreated cells, the distribution of RBM45 is diffuse and nuclear, while the distribution of G3BP is diffuse and cytoplasmic. (b-g) Treatment with the indicated stressors induced the robust formation of G3BP-positive SGs, which were negative for RBM45. RBM45-positive nuclear stress bodies form in response to each stressor. Arsenite = 1 mM arsenite for 1 h, Heat Shock = 42 °C for 1 h, Serum Dep = serum deprivation for 2 h, CdSO4 = 30 μM cadmium sulfate for 2 h, MTX = 20 μM mitoxantrone for 6 h.

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National Institute of Neurological Disorders and Stroke

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