13075_2021_2597_MOESM1_ESM.tiff (3.63 MB)
Additional file 1 of Pathogenic roles and therapeutic potential of the CCL8–CCR8 axis in a murine model of IgG4-related sialadenitis
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posted on 2021-08-17, 10:34 authored by Fumika Honda, Hiroto Tsuboi, Yuko Ono, Saori Abe, Hiroyuki Takahashi, Kiyoaki Ito, Kazunori Yamada, Mitsuhiro Kawano, Yuya Kondo, Kenichi Asano, Masato Tanaka, Marie Malissen, Bernard Malissen, Isao Matsumoto, Takayuki SumidaAdditional file 1: Figure S1. Flow cytometric analysis of spleen and SGs for cytokine production. (A) Gating strategy for flow cytometric analysis of CD4+ T cells. Images are representative results of splenocyte from littermate mice. (B) FACS plot for production of IFN-γ, IL-17, and IL-4 by CD4+ cells derived from 6-week-old littermates and LAT mice after stimulation with phorbol myristate acetate and ionomycin for 4 hours. CD4+ gated cells were shown in FACS plot. FACS plot is a representative of analysis of six mice (littermate, n=3; LAT, n=3).
