Additional file 1 of Ocular macrophage origin and heterogeneity during steady state and experimental choroidal neovascularization
figureposted on 2020-11-14, 04:33 authored by Steven Droho, Benjamin R. Thomson, Hadijat M. Makinde, Carla M. Cuda, Harris Perlman, Jeremy A. Lavine
Additional file 1: Figure S1. Gating strategy and analysis of peripheral blood in male MacGFP mice. (A) Gating strategy for the identification of singlet, CD45+ cells are shown across the top. B cells (CD19+, bottom right), T cells (CD4/CD8+, bottom right), NK Cells (CD11b+NK1.1+, bottom middle), eosinophils (CD11b+SSC-H+Ly6G-), and neutrophils (CD11b+Ly6G+) are delineated. Monocytes are identified from the CD11b+Ly6G-SSC-HLow group (Not PMNs) as CD115+ and either GFP+ or GFP- from oil (B), tamoxifen (Tam) Early (1 week, C), or Tam Late (4-6 weeks, D). Quantitative analysis of monocytes (E), NK cells (F), T cells (G), eosinophils (H), neutrophils (I), and B cells (H). * p < 0.05, *** p < 0.001. GFP+ cells were compared using the Brown-Forsythe and Welch ANOVA followed by Dunnett’s T3 multiple comparison.