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Additional file 1 of Ocular macrophage origin and heterogeneity during steady state and experimental choroidal neovascularization

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posted on 2020-11-14, 04:33 authored by Steven Droho, Benjamin R. Thomson, Hadijat M. Makinde, Carla M. Cuda, Harris Perlman, Jeremy A. Lavine
Additional file 1: Figure S1. Gating strategy and analysis of peripheral blood in male MacGFP mice. (A) Gating strategy for the identification of singlet, CD45+ cells are shown across the top. B cells (CD19+, bottom right), T cells (CD4/CD8+, bottom right), NK Cells (CD11b+NK1.1+, bottom middle), eosinophils (CD11b+SSC-H+Ly6G-), and neutrophils (CD11b+Ly6G+) are delineated. Monocytes are identified from the CD11b+Ly6G-SSC-HLow group (Not PMNs) as CD115+ and either GFP+ or GFP- from oil (B), tamoxifen (Tam) Early (1 week, C), or Tam Late (4-6 weeks, D). Quantitative analysis of monocytes (E), NK cells (F), T cells (G), eosinophils (H), neutrophils (I), and B cells (H). * p < 0.05, *** p < 0.001. GFP+ cells were compared using the Brown-Forsythe and Welch ANOVA followed by Dunnett’s T3 multiple comparison.


National Eye Institute Lupus Research Alliance National Institute of Arthritis and Musculoskeletal and Skin Diseases National Heart, Lung, and Blood Institute National Institute on Aging United States - Israel Binational Science Foundation Rheumatology Research Foundation