Additional file 1: of Nervous system development in the Pacific oyster, Crassostrea gigas (Mollusca: Bivalvia)

Figure S1. Ciliation in Crassostrea gigas visualized with anti-acetylated tubulin. a and b: External ciliation. c-e: Optical sections through the middle of the larval body. a: Ciliated blastula stage. b: The trochophore stage with a prominent prototroch (pt) ring. A presumptive mouth opening (pmo) is located on the ventral side underneath the prototroch. c: The late trochophore stage possesses a well-developed prototroch and telotroch, and the digestive system consists of a ciliated mouth (m), esophagus (es), and a digestive mass (DM) as an anlagen of the stomach. c: The early veliger stage is the first feeding stage with a well-developed digestive system including a ciliated mouth (m), esophagus (es), differentiated stomach (st), intestine (in), and anus (a). The differentiated foot (f) is located between the mouth and anus. e: Ciliation in the late veliger is similar to that in the previous larval stage. Scale bar = 20 μm. (TIFF 2302 kb)