Additional file 1: of G-quadruplex ligand RHPS4 radiosensitizes glioblastoma xenograft in vivo through a differential targeting of bulky differentiated- and stem-cancer cells

Figure S1. DNA damage induction observed in U251MG-derived tumors after RHPS4 in vivo treatment. Graphical representation of the experimental procedure. (A) Immunohistochemical analysis of the neural marker GFAP in a tumor section recovered from the flank of an U251MG injected mouse. (B) Immunostaining of the DNA damage marker 53BP1 in tumor sections recovered from mice exposed 10 mg/Kg/die RHPS4 for either 5 or 10 days and in matched controls (only vehicle). (C) Analysis of either 53BP1 foci/cell or frequency of cells positive to 53BP1 (cells that display > 4 foci per cell). (D) Black bars denotes s.d. (4 animals analyzed for each treatment condition). * P < 0.05; ** P < 0.01 (Student’s t-test). (TIF 18887 kb)