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Additional file 1 of Blockade of glutamine-dependent cell survival augments antitumor efficacy of CPI-613 in head and neck cancer
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posted on 2021-12-15, 04:36 authored by Liwei Lang, Fang Wang, Zhichun Ding, Xiangdong Zhao, Reid Loveless, Jin Xie, Chloe Shay, Peng Qiu, Yonggang Ke, Nabil F. Saba, Yong TengAdditional file 1: Supplementary Fig. S1. ATP amount in HNSCC cells treated with or without CPI-613 in culture medium supplemented with glucose or pyruvate. HN6 and HN12 cells were cultured in DMEM medium overnight, followed by addition of 100 μM CPI-613 and 25 mM glucose or 5 mM pyruvate. After 24 h of treatment, the amount of ATP in cell lysates was determined by CellTiter-Glo® 2.0 Assay Kit. *p < 0.05; **p < 0.01.
