12885_2016_2767_MOESM1_ESM.tif (696.2 kB)

Additional file 1: of Bicyclol induces cell cycle arrest and autophagy in HepG2 human hepatocellular carcinoma cells through the PI3K/AKT and Ras/Raf/MEK/ERK pathways

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posted on 21.09.2016, 05:00 by Yu Wang, Hao Nie, Xin Zhao, Yong Qin, Xingguo Gong
(A) The DNA distribution of cells treated with bicyclol-siRNA-, bicyclol + siRNA-,bicyclol-AKT siRNA+, bicyclol + AKT siRNA+, bicyclol-ERK siRNA+ and bicyclol + ERK siRNA+. The siRNA was transfected as mentioned in Methods. Then the cells were treated with bicyclol for 24 h. (B) MTT results of HepG2 cell viability with treatment 500 μM Bicyclol or 10 μM Sorafenib. (TIF 696 kb)