Additional file 1: of A novel bladder cancer - specific oncolytic adenovirus by CD46 and its effect combined with cisplatin against cancer cells of CAR negative expression

The basic structure chart of skeleton plasmid Ad5/F11p. According to cutting into pAdEasy-1 gene sequence by restriction enzyme EcoRI and overlap extending fiber gene of Ad5 and Ad11p by polymerase chain reaction method, the fiber gene in the pAdEasy-1 was replaced with a chimeric fiber gene of Ad5/F11p encoding the Ad5 fiber tail domain and Ad11p fiber shaft and knob domains. The yellow arrow area represent the chimeric Ad5/F11p fiber. (TIFF 356 kb)