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13287_2015_125_MOESM1_ESM.png (558.54 kB)

Additional file 1: Figure S1. of ROCK activity and the Gβγ complex mediate chemotactic migration of mouse bone marrow-derived stromal cells

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posted on 2015-07-24, 05:00 authored by Caroline Ryan, James Brown, Emer Bourke, Áine Prendergast, Claire Kavanagh, Zhonglin Liu, Peter Owens, Georgina Shaw, Walter Kolch, Timothy O’Brien, Frank Barry
Stages of BMSCs observed in culture. Permeabilised serum-starved BMSCs immunofluorescently stained with F-actin (red) and α-tubulin (green) and DNA counter-stained with DAPI (blue). Scale bar = 10 μm. Representative BMSC stages observed are immotile, polarised, and actively motile. F-Actin filaments accumulate at the leading edge in polarised BMSCs. Actively motile BMSCs display an accumulation of F-Actin and α-tubulin at the cell edge orientated in the direction of movement. Isotype controls, mouse and rabbit (Thermo Fisher Scientific), demonstrate specificity of staining. BMSC bone marrow-derived stromal cell, DAPI 4′,6-diamidino-2-phenylindole. (PNG 558 kb)