Springer Nature
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Additional file 1: Figure S1. of New ligation independent cloning vectors for expression of recombinant proteins with a self-cleaving CPD/6xHis-tag

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posted on 2017-01-05, 05:00 authored by Marco Biancucci, Jazel Dolores, Jennifer Wong, Sarah Grimshaw, Wayne Anderson, Karla Satchell, Keehwan Kwon
Sixteen representative samples of proteins expressed from vector pCPD show diversity of expression, solubility and recovery after InsP6 induced autoprocessing. Numbers at top indicate CSGID IDP designation as listed in Additional file 2. Lanes for each sample represent Whole cell lysate (L), Soluble fraction from lysate (S), Purified fusion protein (P), and purified protein after CPD autoprocessing (P’). Figure was assembled from multiple SDS-polyacrylamide gels stained with Coomassie Blue. The scores at each stage and the total amount of purified protein after autoprocessing are shown below the gel figure. The amounts of total purified proteins from 1 mL cultures were determined from absorbance at 260 nm using a NanoDrop. (TIFF 830 kb)


National Institute of Allergy and Infectious Diseases