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Additional file 1 of Linc00152 promotes malignant progression of glioma stem cells by regulating miR-103a-3p/FEZF1/CDC25A pathway

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posted on 2022-04-09, 03:22 authored by Mingjun Yu, Yixue Xue, Jian Zheng, Xiaobai Liu, Hai Yu, Libo Liu, Zhen Li, Yunhui Liu
Isolation and identification of GSCs. A. a, c: Cells grew into spheres in serum-free medium. b, d: Spheres generated again from a single cell. B. Individual undifferentiated GSCs-87 and GSCs-251 stained for Nestin (green) and CD133 (red) by immunofluorescence analysis. C. Cell spheres were differentiated and then stained for GFAP (green) and β-tubulin III (red) by immunofluorescence analysis. Nuclei (blue) were labeled with DAPI. Images are representative of independent experiments (n = 5). Scale bars represent 20 μm. (TIFF 35029 kb)


National Natural Science Foundation of China Liaoning Science and Technology Plan Project Shenyang Science and Technology Plan Projects outstanding scientific fund of Shengjing hospital