posted on 2020-10-09, 03:44authored byJan H. Forth, Leonie F. Forth, Samantha Lycett, Lesley Bell-Sakyi, Günther M. Keil, Sandra Blome, Sébastien Calvignac-Spencer, Antje Wissgott, Johannes Krause, Dirk Höper, Helge Kampen, Martin Beer
Additional file 18: Figure S6. The reconstructed ASFV-like A104R protein is highly similar to its ASFV homologue (A) A rabbit antiserum raised against the reconstructed A104 gene recognised a flag-tagged and an untagged version of A104R protein (lanes A104-Flag and A104, respectively), but showed no specific reaction with extracts of tick cell lines OME/CTVM21, OME/CTVM22, OME/CTVM24, and OME/CTVM27. In extracts of WSL-HP cells infected with ASFV Kenya 1033, the serum reacted with a single band of 12 kDa which is similar to the calculated molecular weight of ASFV A104R (11.6 kDa). (B) The Coomassie stained gel confirms equal loading with proteins.
Funding
Biotechnology and Biological Sciences Research Council (GB) Biotechnology and Biological Sciences Research Council Max-Planck-Gesellschaft Horizon 2020 (COMPARE) Bundesministerium für Bildung und Forschung