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Additional file 10: of Probabilistic modeling of personalized drug combinations from integrated chemical screen and molecular data in sarcoma

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posted on 2019-06-17, 05:00 authored by Noah Berlow, Rishi Rikhi, Mathew Geltzeiler, Jinu Abraham, Matthew Svalina, Lara Davis, Erin Wise, Maria Mancini, Jonathan Noujaim, Atiya Mansoor, Michael Quist, Kevin Matlock, Martin Goros, Brian Hernandez, Yee Doung, Khin Thway, Tomohide Tsukahara, Jun Nishio, Elaine Huang, Susan Airhart, Carol Bult, Regina Gandour-Edwards, Robert Maki, Robin Jones, Joel Michalek, Milan Milovancev, Souparno Ghosh, Ranadip Pal, Charles Keller
Figure S10. Schematic of PTIM-informed U23674 rewiring experiment. An initial culture of U23674 is screened using the Roche screen. The same culture is used to seed 6 new cultures, which are grown until the cell population is sufficient for drug screening. Five of the 6 cultures were treated using single agents and drug combinations in low dosages (75 nM OSI-906, 50 nM GDC-0941) and one culture was left untreated. After treatment and incubation for 72 h, the compounds were removed the cells were screened using the Roche Orphan Kinome screen. (TIF 8496 kb)


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