Additional file 10: of M6A-mediated upregulation of LINC00958 increases lipogenesis and acts as a nanotherapeutic target in hepatocellular carcinoma

Figure S5. LINC00958 promotes lipogenesis through miR-3619-5p. (A) Effects of miR-3619-5p overexpression on cholesterol level in LINC00958-overexpressed Hep3B cells. The data are shown as the mean ± SEM. ***P < 0.001 vs. the NC group. (B) Effects of miR-3619-5p overexpression on triglyceride level in LINC00958-overexpressed Hep3B cells. The data are shown as the mean ± SEM. ***P < 0.001 vs. the NC group. (C) RT-qPCR assays were used to examine the effects of miR-3619-5p overexpression on SREBP1 level in LINC00958-overexpressed Hep3B cells. The data are shown as the mean ± SEM. ***P < 0.001 vs. the NC group. (D) Western blotting was performed to investigate the effects of miR-3619-5p overexpression on SREBP1 level in LINC00958-overexpressed Hep3B cells. (E) Oil Red O staining showing the effects of miR-3619-5p overexpression on lipid droplet formation in LINC00958-overexpressed Hep3B cells.