Metadata record for the manuscript: Targeting nucleotide metabolism enhances the efficacy of anthracyclines and anti-metabolites in triple-negative breast cancer

Summary

This metadata record provides details of the data supporting the claims of the related manuscript: “Targeting nucleotide metabolism enhances the efficacy of anthracyclines and anti-metabolites in triple-negative breast cancer”.

The related study sought to identify and characterise new therapeutic opportunities to enhance current standard of care (SoC) chemotherapies that incorporate anthracyclines and anti-metabolite 5-Fluorouracil (5-FU) in triple-negative breast cancer (TNBC) through further modulation of pyrimidine and uracil nucleotide metabolism pathways, hypothesising that this could be achieved through inhibition of the gatekeeper enzyme, deoxyuridine 5’-triphosphate nucleotidohydrolase (dUTPase), as this enzyme functions to prevent uracil misincorporation into DNA.

Type of data: cell survival, growth inhibition, DNA damage analysis, apoptosis, nucleotide pool and in vivo data, Western Blot images

Subject of data: MDA-MB-231 (human, ATCC HTB-26), MDA-MB-468 (human, ATCC HTB-132); Female Balb/c mice; 4-6 weeks old; purchased from Envigo.

Sample size: Groups of 5-6 mice for control and experimental conditions were used. Power calculations were used to determine sample size.

Data access

All the data underlying figures 1-8 of the related article, including cell survival, growth inhibition, DNA damage analysis, apoptosis, nucleotide pool and in vivo data, along with data underlying supplementary figures 1, 2, 3, 4, 5, 7, 8 and Western Blot images for supplementary figure 1a-b, are openly available as part of this figshare data record. Data files are named in-line with the figure they underlie, and each may contain multiple tabs for the sub-figures.

Corresponding author(s) for this study

Melissa J LaBonte, Medicine, Dentistry and Biomedical Sciences: Patrick G Johnston Centre for Cancer Research, Queen’s University Belfast, Belfast, BT9 7AE, UK. el: +44 2890972789; Email: m.labontewilson@qub.ac.uk.

Study approval

The mouse work was performed under an approved UK project license and approved by the institutional Queen's University Belfast Animal Welfare Ethical Review Body (AWERB).