12870_2019_2047_MOESM8_ESM.xlsx (19.28 kB)
MOESM8 of Silencing of one copy of the translation initiation factor eIFiso4G in Japanese plum (Prunus salicina) impacts susceptibility to Plum pox virus (PPV) and small RNA production
dataset
posted on 2019-10-23, 08:24 authored by Julia Rubio, Evelyn Sánchez, David Tricon, Christian Montes, Jean-Philippe Eyquard, Aurélie Chague, Carlos Aguirre, Humberto Prieto, Véronique DecroocqAdditional file 8: Table S2. Stem-loop reverse transcription-polymerase chain reaction (RT-PCR) detection of selected siRNAs and miRNAs. * Number indicates the number of reads issued from NGS for each siRNA molecule as depicted in Fig. 3. Loop primer: lower case fonts indicate complementary residues in the loop; bold, italic upper case indicate hybridizing sites for the reverse primer; double underlined fonts, residues hybridizing with the small RNA. Forward primer: double underlined fonts indicate residues hybridizing with the small RNA. wt: non-transformed, wild type ‘Angeleno’; AG7303–96: PpeIFiso4G11-silenced, transgenic ‘Angeleno’ Japanese plants.