MOESM5 of Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects

Additional file 5: Table S1. Summary of the analysis of the potential off-target loci. The top 10 potential off-target loci are PCR amplified and subsequently subjected to Sanger sequencing and aligned with mouse genome. Although no off-target effects of #2–10 loci are found on all the 4 mice, the off-target effects of #1 locus are detected in KI1 and KI3 mice.