MOESM4 of Can supernatant from immortalized adipose tissue MSC replace cell therapy? An in vitro study in chronic wounds model

Additional file 4. Migration activity of native HATMSC supernatants. MSU-1.1 cell migration activity was investigated at 37 °C in an incubation chamber (PeCon GmbH, Erbach, Germany) with 1%O2, 5%CO2 mounted on an Axio Observer inverted microscope equipped with a dry 5x objective (Zeiss, Gottingen, Germany). The movement of the cells was time-lapse recorded for 44 h at intervals of 2 h using Zen 2.6 Blue Edition Software (Zeiss, Gottingen, Germany) as 6 separate movies (one for each supernatant and control).