Additional file 2. Â List of regulated genes in juveniles and adults in response response to OsHV-1, V. aestuarianus and V . tasmaniensis LGP32. Statistical analysis of qPCR data was performed separately for juvenile and adult oysters. Two-way analysis of variance (ANOVA) was conducted to individually assess expression levels of the 102 target genes using the univariate general linear model (GLM) with post hoc Tukeyâs HSD test in IBM SPSS Statistics v 20.0.