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Kallisto output

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posted on 2020-06-17, 10:42 authored by Raymond A Enke, Scott T Schumacker, Krista R Coppage
This data set contains trimmed and psudoaligned sequence files and transcript quantifications generated by the kallisto application for each experimental sample. Kallisto produces three output files per sample: 1. An abundances.h5 HDF5 binary file containing run info, abundance esimates, bootstrap estimates, and transcript length information length. This file can be read in by the sleuth program. 2. An abundances.tsv plaintext file of the abundance estimates. Please use the --plaintext mode to output plaintext abundance estimates. Alternatively, kallisto h5dump can be used to output an HDF5 file to plaintext. The first line contains a header for each column, including estimated counts, TPM, effective length. 3. A run_info.json file containing information about the run. This data set contains an abundances.h5, an abundances.tsv, and a run_info.json file for each of the 37 samples in our study.

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