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12864_2021_8266_MOESM9_ESM.csv (21.23 kB)

Additional file 9 of Extensive evaluation of ATAC-seq protocols for native or formaldehyde-fixed nuclei

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posted on 2022-03-17, 04:30 authored by Hao Zhang, Michael E. Rice, Joseph W. Alvin, Dominique Farrera-Gaffney, James J. Galligan, Michael D. L. Johnson, Darren A. Cusanovich
Additional file 9: Supplementary Table S9. List of ATAC-seq primers used for PCR and corresponding samples for each barcode. The Nextera N5 primer (Ad1_noMX) and the N7 primer “Ad2.1” and other N7 primer sequences (besides the barcodes) were obtained from [5]. Column 1 (“Name”) indicates the name we used for each primer. Column 2 (“Barcode in Fastq”) shows the sequence returned by the sequencer for that particular primer. Column 3 (“Oligo”) shows the sequence of the primers. Columns 4-11 specify the sample ID, buffer, transposition temperature, enzyme source, fixation state, nuclei input, replicate ID, and sample type for each sample indexed by the corresponding barcode.