posted on 2015-12-26, 05:00authored byRuud Weijer, Mans Broekgaarden, Rowan van Golen, Esther Bulle, Esther Nieuwenhuis, Aldo Jongejan, Perry Moerland, Antoine van Kampen, Thomas van Gulik, Michal Heger
Overview of primer sequences and resulting amplicon sizes (in bp) that were used for the validation of microarray expression levels using qRT-PCR. The primers are listed in the 5Ⲡto 3Ⲡdirection. Primers were derived from RTPrimerDB ( http://www.rtprimerdb.org/ ), NCBI primer Blast ( http://www.ncbi.nlm.nih.gov/tools/primer-blast/ ), and qPrimerDepot ( http://primerdepot.nci.nih.gov/ ). Sequencing of the qRT-PCR products, which were analyzed using NCBI Blast ( http://blast.ncbi.nlm.nih.gov/Blast.cgi ), confirmed the correct identity of each product. (XLSX 14 kb)