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12864_2017_3745_MOESM4_ESM.xlsx (15.58 kB)

Additional file 4: of The dynamics of early-state transcriptional changes and aggregate formation in a Huntington’s disease cell model

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posted on 2017-05-12, 05:00 authored by Martijn van Hagen, Diewertje Piebes, Wim de Leeuw, Ilona Vuist, Willeke van Roon-Mom, Perry Moerland, Pernette Verschure
The table shows the mean log2-ratio with respect to the Cy5-labelled common reference of all probes used to measure eGFP-Htt expression levels, i.e. eGFP-82, eGFP-162, Linker, HTT, HTT repeat, eGFP-82 rc, eGFP-162 rc, Linker rc, HTT rc, HTT repeat rc (Fig. 2c; rc: reverse complementary) and also the probe targeting endogenous Htt (ENSRNOG00000011073) upon doxycline induction in the Q23 control and Q74 Htt cell line. The expression values of these probes were pre-processed along with all other probes on the microarrays and the fold changes in eGFP-Htt expression with respect to the pre-induction state (0 h) in control and HD cells were calculated for each time point as shown in Fig. 2d, e. (XLSX 15 kb)