12915_2021_1108_MOESM4_ESM.xlsx (1.11 MB)
Additional file 4 of Mutant resources for functional genomics in Dictyostelium discoideum using REMI-seq technology
dataset
posted on 2021-08-25, 03:35 authored by Nicole Gruenheit, Amy Baldwin, Balint Stewart, Sarah Jaques, Thomas Keller, Katie Parkinson, William Salvidge, Robert Baines, Chris Brimson, Jason B. Wolf, Rex Chisholm, Adrian J. Harwood, Christopher R. L. ThompsonAdditional file 4. Table of individual mutants present in the REMI grid. Individual mutants were identified in 96-well plates using the REMI-seq technique (see methods). To identify the exact insertion points, the extracted tags were compared to the lookup table. The unique indices at the end of the inserts also allowed the determination of the vector version (p-GWDI G1 – G5; C6 – C8) as well as the orientation of the insert.