12896_2020_626_MOESM4_ESM.xls (2.31 MB)
Additional file 4 of Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system
dataset
posted on 2020-06-24, 03:36 authored by Remi L. Gratacap, Tim Regan, Carola E. Dehler, Samuel A. M. Martin, Pierre Boudinot, Bertrand Collet, Ross D. HoustonAdditional file 4 Table S1. Predicted Off-target sites. List of all predicted off-targets from CRISPOR website for 354 bp in RIG-I exon 2 (Tab1). The selected gRNA is labeled 224 forw and the list of off-targets can be found lines 2127–2240. Tab2 summarises the predicted off-targets for gRNA 224 forw. The sum of the two off-target scores (MIT and CDF) were calculated to rank the results. The 2 targets selected are highlighted.
