Additional file 4: Figure S4. of Inhibition of Oct 3/4 mitigates the cardiac progenitor-derived myocardial repair in infarcted myocardium

Showing apoptotic signals from CSC-engrafted myocardium. A Representative immunostaining images of apoptosis in post-MI myocardium. Cardiomyocytes that underwent apoptosis were identified by active caspase-3 (green). CSC-derived cardiomyocytes were stained with GFP (red). DAPI was used to visualize nuclei. Scale bar: 50 μm. B GFP-positive apoptotic cells in MI heart that received CSC engraftment. The unit was normalized to the cell number per mm2. The primary antibodies that were used in this study include the following: engrafted CSCs were identified with mouse monoclonal GFP (Clontech, Mountain View, CA, USA) and costained with polyclonal active caspase 3 (1:100; Abcam, Cambridge, MA, USA). The methodology for immunostaining is identical to the described description in Materials and methods. Value represent mean ± SE (n = 3 hearts per group). (ZIP 271 kb)