posted on 2023-04-13, 15:06authored byWilliam Poisson, Julien Prunier, Alexandra Carrier, Isabelle Gilbert, Gabriela Mastromonaco, Vicky Albert, Joëlle Taillon, Vincent Bourret, Arnaud Droit, Steeve D. Côté, Claude Robert
Additional file 3. Primers and detection oligo. Excel file listing the primers used for amplification and transcription reactions and as detection oligo. Reverse primers consist of the 20-mers complementary sequence linked to the 3’ end of the T7 sequence 5’-CGATTGAGGCCGGTAATACGACTCACTATAGGG-3’ [45]. Forward primer 20-mers are linked to the 3’ end of the adapter sequence. The resulting 53-mers reverse primer and 40-mers forward primer oligo were used for PCR. Forward primers were used also for RT-PCR. The fluorophore-linked homolog of the specific 20-mers adapter was the detector of probe binding. These 30 oligo were purchased from IDT as custom standard or HPLC-purified DNA.
Funding
Génome Québec Genome Canada Natural Sciences and Engineering Research Council of Canada Fonds de recherche du Québec – Nature et technologies