Additional file 2 of Short- and long-term dietary supplementation as well as withdrawal of the enteric methane inhibitor 3-nitrooxypropanol reveal distinct effects on the rumen microbial community

Additional file 3: Fig. S1. Rarefaction curves of bacterial, archaeal, and protozoal communities after short- and long-term 3-NOP supplementation in beef cattle. Fig. S2. Comparison of alpha (Shannon index) and beta (Bray–Curtis dissimilarity) diversities of bacterial, archaeal, and protozoal communities in short-term (ST) and long-term (LT) periods. Fig. S3. Venn diagrams showing the genera of rumen microbes shared between and unique to short- and long-term studies. Fig. S4. Comparison of rumen microbial taxa after short-term 3-NOP supplementation in beef cattle. Fig. S5. Comparison of rumen microbial taxa between control and high groups after long-term 3-NOP supplementation in beef cattle. Fig. S6. Comparison of rumen microbial taxa between control and high-R* (recovery) groups after long-term 3-NOP supplementation in beef cattle. Fig. S7. Comparison of rumen microbial taxa between high and high-R* (recovery) groups after long-term 3-NOP supplementation in beef cattle. Fig. S8. Comparison of rumen microbial taxa after 3-NOP supplementation in beef cattle in short- and long-term periods. Fig. S9. Differentially abundant predicted microbial functions associated with methane metabolism after short-term 3-NOP supplementation in beef cattle. Fig. S10. Differentially abundant predicted microbial functions associated with methane metabolism after long-term 3-NOP supplementation in beef cattle. Fig. S11. Topological roles of rumen microbial taxa derived from co-occurrence networks after short-term and long-term 3-NOP supplementation.