Springer Nature
Browse

Additional file 2 of Identification of African swine fever virus-like elements in the soft tick genome provides insights into the virus’ evolution

Download (14.68 kB)
dataset
posted on 2020-10-09, 03:44 authored by Jan H. Forth, Leonie F. Forth, Samantha Lycett, Lesley Bell-Sakyi, Günther M. Keil, Sandra Blome, Sébastien Calvignac-Spencer, Antje Wissgott, Johannes Krause, Dirk Höper, Helge Kampen, Martin Beer
Additional file 2: Table S2. ASFV-like transcripts detected in data from six Ornithodoros moubata cell lines and O.moubata ticks by mRNA-sequencing and data analysis from published sequences ASFV-like sequences from tick cell libraries lib01543-46 und lib01610-11 (total RNA) were identified using RIEMS. The resulting reads were blasted against the NCBI BLASTn database for confirmation and annotation. Ornithodoros moubata transcriptome data from a previous study42 was downloaded from GenBank, and ASFV-like sequences were identified by mapping the data against a tailored database containing all known ASFV sequences and all previously obtained ASFV-like sequences. The resulting reads and contigs were annotated after a BLASTn search against the entire BLASTn database. Sequencing data from the mRNA enriched OME21 library lib02965 was mapped against a tailored database containing all known ASFV sequences and all previously obtained ASFV-like sequences, and resulting contigs were blasted against the entire BLASTn database. Per sequence, only the hit with the lowest e-value is shown.

Funding

Biotechnology and Biological Sciences Research Council (GB) Biotechnology and Biological Sciences Research Council Max-Planck-Gesellschaft Horizon 2020 (COMPARE) Bundesministerium für Bildung und Forschung

History

Usage metrics

    BMC Biology

    Categories

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC