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Additional file 14 of Intradiol ring cleavage dioxygenases from herbivorous spider mites as a new detoxification enzyme family in animals

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posted on 2022-06-06, 07:58 authored by Christine Njiru, Wenxin Xue, Sander De Rouck, Juan M. Alba, Merijn R. Kant, Maksymilian Chruszcz, Bartel Vanholme, Wannes Dermauw, Nicky Wybouw, Thomas Van Leeuwen
Additional file 14: Table S9. Enzyme characteristics. Protein yields were estimated from protein concentrations determined using Bradford assay. Iron content was estimated using ferrozine assay with a self-developed Fe3+ standard curve. All purified DOG proteins had good enough yields, with the lowest yields in PcDOG5 and PcDOG6. All proteins were able to bind varying amounts of iron. The errors in iron content represent standard deviation of the mean (n=4).

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European Union Horizon 2020 Marie Skłodowska-Curie Action Individual Fellowship BOF fellowship USDA's National Institute of Food and Agriculture

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